Pre-cementation procedures' effect on dental zirconias with different yttria content.

Objectve: Several pre-cementation procedures have been advocated to enhance adhesion between zirconia and resin-based cement. There is, however, limited documentation on how these pre-treatments affect the strength of zirconia crowns as most tests are performed on discs or bars. The aim was to assess the effect of pre-cementation procedures on fracture mode, fracture strength and cement retention on zirconia.

FRET-based cyclic GMP biosensors measure low cGMP concentrations in cardiomyocytes and neurons.

Several FRET (fluorescence resonance energy transfer)-based biosensors for intracellular detection of cyclic nucleotides have been designed in the past decade. However, few such biosensors are available for cGMP, and even fewer that detect low nanomolar cGMP concentrations. Our aim was to develop a FRET-based cGMP biosensor with high affinity for cGMP as a tool for intracellular signaling studies.

Preassociation between the 5-HT serotonin receptor and G protein G: molecular determinants and association with low potency activation of adenylyl cyclase.

According to early models of GPCR signaling, G proteins only interact with activated receptors. However, some GPCRs were shown to assemble with G proteins before receptor activation, in accordance with more recent models. Previously, we found that the 5-HT receptor, as opposed to the 5-HT receptor, was preassociated with G, but the molecular determinants for this interaction are still elusive.

Related GPCRs couple differently to G: preassociation between G protein and 5-HT serotonin receptor reveals movement of Gα upon receptor activation.

How GPCRs and G proteins interact is important for their biologic functions and their functions as pharmacologic targets. It is still an open question whether receptors and G proteins are preassembled in a complex or interact only after receptor activation. We compared the propensity of the two G-coupled serotonin (5-HT) receptors 5-HT and 5-HT to associate with G protein prior to agonist activation.