Publications by authors named "A H L Mulder"
Expression of CD2, CD25 and/or CD30 in extracutaneous mast cells (MC) is a minor diagnostic criterion for systemic mastocytosis (SM) in the classification of the World Health Organization and International Consensus Classification. So far, it remains unknown whether expression of these antigens on MC is of prognostic significance in SM. We performed a retrospective multi-center study of patients with SM using the data set of the registry of the European Competence Network on Mastocytosis, including 5034 patients with various MC disorders.
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- Whole genome sequencing (WGS) of Mycobacterium tuberculosis complex (MTBC) is greatly affected by the effectiveness of DNA extraction protocols, particularly the lysis step.
- In a study comparing four different DNA extraction methods, a bead beating protocol (protocol B) significantly increased DNA yield by 60-fold and improved sequencing success rates.
- Incorporating mechanical lysis not only enhances extraction from primary cultures but also enables earlier WGS from enriched cultures, ultimately reducing turnaround time while maintaining high-quality DNA needed for analysis.
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- Giant cell arteritis (GCA) and polymyalgia rheumatica (PMR) are inflammatory diseases, and researchers are looking for biomarkers to identify active cases and those who may need alternative treatments to glucocorticoids.
- A study evaluated the presence of interferon type I (IFN-I) markers in GCA and PMR patients, including those not previously treated, but did not find significant differences in IFN-I scores between these groups and healthy controls.
- The results indicated that the IFN-I signature and serum markers CXCL10 and Galactin-9 were not elevated in patients with GCA or PMR, suggesting that these IFN-I markers may not be useful for diagnosing or guiding treatment
Defining what constitutes a healthy microbiome throughout our lives remains an ongoing challenge. Understanding to what extent host and environmental factors can influence it has been the primary motivation for large population studies worldwide. Here, we describe the fecal microbiome of 3,746 individuals (0-87 years of age) in a nationwide study in the Netherlands, in association with extensive questionnaires.
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- A 42-year-old man experienced renal failure and ongoing fatigue after a SARS-CoV-2 infection, leading to referral to internal medicine.
- Blood tests showed he had anemia and severe kidney issues, and further testing identified type I cryoglobulinemia with antibodies targeting SARS-CoV-2.
- The patient's symptoms and lab results improved over three months, suggesting that COVID-19 induced cryoglobulinemia is typically mild and resolves on its own after the viral infection clears.