Publications by authors named "A H GROTH"

Objectives: Home-based hematopoietic stem cell transplantation (HCT) is a novel approach that has the potential to improve outcomes, however, the impact of transplant location on the gut microbiome remains uncharacterized. We hypothesized that patients randomized to undergo home HCT would have higher gut taxonomic diversity and lower antimicrobial resistance (AMR) gene abundance compared to those undergoing standard hospital HCT.

Methods: We identified 28 patients enrolled in Phase II randomized trials of home (n=16) v.

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We present replication-aware single-molecule accessibility mapping (RASAM), a method to nondestructively measure replication status and protein-DNA interactions on chromatin genome-wide. Using RASAM, we uncover a genome-wide state of single-molecule "hyperaccessibility" post-replication that resolves over several hours. Combining RASAM with cellular models for rapid protein degradation, we demonstrate that histone chaperone CAF-1 reduces nascent chromatin accessibility by filling single-molecular "gaps" and generating closely spaced dinucleosomes on replicated DNA.

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Article Synopsis
  • Long-term issues in chromatin assembly during DNA replication can significantly impact how cells maintain their epigenetic information and decide their fate.
  • Researchers studied the effects of quickly removing a key protein called CAF-1, which is crucial for building chromatin, by using advanced techniques like single-cell genomics and live microscopy.
  • The loss of CAF-1 slows DNA replication, makes new DNA more accessible, triggers a unique cellular response that reduces histone mRNA levels, and ultimately leads to cell-cycle arrest influenced by p53, highlighting the immediate consequences of faulty chromatin assembly.
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Article Synopsis
  • Recent advancements in aging research and drug discovery connect basic research with clinical applications, aiming to promote healthy longevity in humans.* -
  • The Aging Research and Drug Discovery Meeting in 2023 highlighted key areas such as AI, biomarkers, geroscience, and clinical trials focused on enhancing healthspan.* -
  • The meeting emphasized the importance of combining generative AI with innovative biological technologies to tackle age-related diseases and extend healthy lifespans.*
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