One Size Fits Small: The Narrow Utility for Plasma Metagenomics.

Metagenomic sequencing of plasma has been advertised by Karius, Inc. as a way to diagnose a variety of infectious syndromes. Due to the lack of robust evidence of clinical utility, our laboratory began actively stewarding Karius testing.

Oxygen uptake efficiency slope at anaerobic threshold can predict peak VO in adult congenital heart disease.

Introduction: Assessment of exercise capacity by cardiopulmonary exercise testing (CPET) in adults with congenital heart disease (CHD) is important for prognostication and preoperative assessment. Peak oxygen uptake (PVO) is used commonly, but can be challenging due to the difficulties of undertaking maximal CPET testing in this population. We explored whether oxygen uptake efficiency slope (OUES) at ventilatory anaerobic threshold (VAT), the point during CPET at which OUES becomes strongly correlated with PVO, and is more reliably available from submaximal CPET, can predict PVO in adults with CHD.

Trace elements in Alaska's ice seals in the 2000s and 2010s.

Ringed (Pusa hispida), bearded (Erignathus barbatus), spotted (Phoca largha), and ribbon (Histriophoca fasciata) seals are ice-associated seals that are important subsistence resources for coastal Alaska Native people. These seals are also mid- to upper trophic level Arctic predators and primary prey of polar bears (Ursus maritimus). We analyzed concentrations of 19 trace elements in seal liver, kidney, muscle, and blubber, including arsenic, cadmium, lead, mercury, and vanadium due to their potential toxicity.

Military spouse and key stakeholder perspectives of effective messaging for US service members on secure storage of personal firearms: a qualitative study.

Background: Secure firearm storage is a proposed method for reducing intentional and unintentional firearm injury and death among US military service members. However, little is known about suggested key messengers and optimal message content to promote secure firearm storage practices among at-risk US service members. This qualitative study focused on military spouse and stakeholder perspectives concerning key messengers and message content for the delivery of effective messaging around promoting secure firearm storage practices among US service members.

Estimating Demographic Parameters for Bearded Seals, , in Alaska Using Close-Kin Mark-Recapture Methods.

Reliable estimates of population abundance and demographics are essential for managing harvested species. Ice-associated phocids, "ice seals," are a vital resource for subsistence-dependent coastal Native communities in western and northern Alaska, USA. In 2012, the Beringia distinct population segment of the bearded seal, , was listed as "threatened" under the US Endangered Species Act requiring greater scrutiny for management assessments.