Detection of apoptosis by the TUNEL technique in clinically localised prostatic cancer before and after combined endocrine therapy.

Aims: Apoptosis in prostate cancer was evaluated after three months of combined endocrine therapy to investigate the association with tumour grade, tumour stage, and the immunohistochemical detection of p53 and bcl-2 in tumour cells before and after therapy.

Methods: Twenty six formalin fixed, paraffin wax embedded core biopsies and corresponding prostatectomy specimens, excised after three months of combined endocrine therapy, were analysed for the presence of apoptotic cells by the terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end labelling (TUNEL) method, and for p53 and bcl-2 overexpression by immunohistochemistry.

Results: All 26 adenocarcinomas were clinically localised at diagnosis.

[Our experience with prostatic incision (TUIP) with local anesthesia].

The Authors present their experience in the treatment of prostatic obstruction with bladder neck incision (TUIP) performed under local anesthesia. An Hulbert 6 Fr endoscopic needle is used to infiltrate the prostatic area submitted to TUIP with 200 mg of Lidocaine 2%. The TUIP was done with a single deep incision at 7 hours using a 24 Fr Iglesias resector with Collins device.

[Our experience concerning scrotal traumatology].

The Authors present their experience on 24 blunt scrotal traumas observed since January 1991. They underline the importance of an ecographic scanning that enables a precise evaluation of the traumatic event. Patients that present positive ultrasonic findings are promptly operated thus permitting bleeding and infection control.

[Our technique in orthotopic neobladder].

The strive in the field of orthotopic neobladders derives from the need to improve their morphofunctional aspects and to simplify the surgical procedures. The Authors propose their experience on a new method of orthotopic neobladder in 8 patients submitted to radical cystectomy for advanced bladder neoplasia from march 91 until june 93. The surgical technique was to prepare a reservoir with a simile Camey 2 type procedure modified by them using 50 cm of ileus, 30 of which detubularized and reconfigured into a simile spheric shape with Polygia 75 staplers.

[Transperitoneal nephrectomy of crossed ectopic kidney without fusion associated with stenosis of the pyeloureteral junction and pyelocaliceal staghorn calculi].

The authors report their experience on a case of crossed ectopic kidney without fusion associated with stenosis of the pyeloureteral junction and secondary pyelocaliceal staghorn calculosis. After a brief summary of the various types of kidney ectopia and related principles of operation they describe their case report underlying the rarity of their case and the surgical approach. They consider the anterior transperitoneal approach as the most indicated for this type of malformation as it permits a better surgical view of the kidney and of the vascular structures.