Publications by authors named "A Gruez"
Article Synopsis
- The study focuses on how enzymes, specifically HMG-CoA synthase (HMGCS), have evolved to take on new roles in polyketide biosynthesis, which involves a different substrate interaction than their traditional function.
- Researchers used techniques like X-ray crystallography and small-angle X-ray scattering to show that an HMGS from the virginiamycin system is more flexible compared to its typical counterparts, which is crucial for its ability to handle larger substrates.
- Findings highlight the importance of combining different structural biology methods since existing models like AlphaFold2 failed to accurately predict the enzyme's structural transitions when binding to its natural substrates.
Article Synopsis
- Pyrrolizidine alkaloids (PAs) are a diverse group of compounds found in plants and bacteria, characterized by a specific chemical structure and produced through two main pathways (one in plants and another in bacteria).
- The study identified a gene cluster in the bacterium Xenorhabdus hominickii responsible for the production of a specific PA called pyrrolizwilline, shedding light on its biosynthesis.
- Researchers also characterized an important enzyme in the pathway, XhpG, utilizing advanced techniques like X-ray crystallography to understand its role in converting a precursor compound into pyrrolizwilline.
Modular trans-acyltransferase polyketide synthases (trans-AT PKSs) are enzymatic assembly lines that biosynthesize complex polyketide natural products. Relative to their better studied cis-AT counterparts, the trans-AT PKSs introduce remarkable chemical diversity into their polyketide products. A notable example is the lobatamide A PKS, which incorporates a methylated oxime.
View Article and Find Full Text PDFDuring biosynthesis by multi-modular trans-AT polyketide synthases, polyketide structural space can be expanded by conversion of initially-formed electrophilic β-ketones into β-alkyl groups. These multi-step transformations are catalysed by 3-hydroxy-3-methylgluratryl synthase cassettes of enzymes. While mechanistic aspects of these reactions have been delineated, little information is available concerning how the cassettes select the specific polyketide intermediate(s) to target.
View Article and Find Full Text PDFMembers of species complex (FSSC) are cosmopolitan filamentous fungi responsible for invasive fungal infections in immunocompromised patients. Despite the treatment recommendations, many strains show reduced sensitivity to voriconazole. The objective of this work was to investigate the potential relationship between azole susceptibility and mutations in CYP51 protein sequences.
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