Protocol for generating and characterizing a nasal epithelial model using imaging with application for respiratory viruses.

Air-liquid interface (ALI) culture can differentiate airway epithelial cells to recapitulate the respiratory tract in vitro. Here, we present a protocol for isolating and culturing nasal epithelial cells from turbinate tissues for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. We describe steps to overcome challenges of imaging fragile cultures, detect the production of mucus, and quantify intracellular virus post-SARS-CoV-2 infection.

Impacts of microplastic accumulation in aquatic environment: Physiological, eco-toxicological, immunological, and neurotoxic effects.

The presence of microplastics (MPs) in aquatic ecosystem has become a pressing global concern. MPs pose a significant threat to aquatic ecosystems, with devastating consequences for both aquatic life and human health. Notably, freshwater ecosystems are particularly vulnerable to MPs pollution.

Post-traumatic extensive chronic osteomyelitis of skull vault: An illustrative case report.

Chronic osteomyelitis of the skull base is a commonly reported pathology in existing scientific literature, but chronic osteomyelitis of the skull vault (COSV) is a rarely documented disease. We report the case of a 38 years old Afghan male with a presenting complaint of irregular swelling on the skull vault for six months. The patient had a history of head trauma one year back with a compound depressed fracture which had been surgically managed then.

Multiple primary brain abscesses in a 14 years old immunocompetent boy: A tale of Proteus mirabilis infestation from Pakistan.

Brain abscess (BA) formation that may be due to due to Gram-positive bacteria commonly and less commonly due Gram-negative bacteria affects pediatric population. Most cases are secondary to the involvement of ear, nose, throat and sinuses (ENTS). We describe a rare case of a 14 year old patient presenting with generalized tonic clonic seizures for the last six months associated with fever, vomiting and headache.

Construction, molecular characterization, and safety assessment of mutant of Gallinarum.

This study involves the development and molecular characterization of the isogenic markerless knockout mutant SG Δ, a genetically engineered live attenuated strain aimed at controlling Gallinarum (SG) infection in poultry. The mutant was generated by deleting the gene using -Red recombination technology, impairing adenylosuccinate lyase, necessary for purine biosynthesis. An 1,180 bp deletion was engineered within the gene, leaving a residual 298 bp genomic scar resulting in a purine auxotrophic mutant.