Resolving viral structural complexity by super-resolution microscopy.

In this review, we discuss different super-resolution microscopy (SRM) techniques employed to study viral structures and virus composition with nanometric resolution. We describe the basic principles of the different microscopy methods utilized to break the light diffraction limit, enabling the study of protein composition in viral structures. Finally, we demonstrate for the first time the differential spatial distribution of two structural proteins in an individual baculovirus using single-molecule super-resolution microscopy.

Resolving the differential distribution of structural proteins in baculovirus using single-molecule localization microscopy.

Baculovirus is one of the most complex viruses found in nature. Proteomic analysis of budded viruses (BVs) indicates that they are formed by at least 50 different structural proteins. The function of most of these structural proteins and their specific localization in individual virions remain unknown.

The "Emorality" of Caring: Validation of an Empirical Model of the Moral Feelings of Affective Care in Teaching Communities.

This article presents a study that addresses the challenge of establishing a relationship between the axiological and the affective, by validating a structural model through an assessment instrument (SEMORCUNA) that isolates the moral feelings associated with 'affective care'. The research sample consisted of 222 teachers, all of whom were either in training or were active professionals in the teaching field. To achieve the research objectives, a group of experts selected a total of 11 moral sentiments, based on which Principal Component Analysis was conducted.

Mechanically Flexible, Large-Area Fabrication of Three-Dimensional Dendritic Au Films for Reproducible Surface-Enhanced Raman Scattering Detection of Nanoplastics.

The escalating crisis of nanoplastic pollution in water and food products demands the development of novel methodologies for detection and recycling. Despite various techniques available, surface-enhanced Raman scattering (SERS) is emerging as a highly efficient technique for the trace detection of micro/nanoplastics. However, the development of highly reproducible and stable, flexible SERS substrates that can be used for sensitive detection in environmental medium remains a challenge.