Publications by authors named "A G Mikheeva"
Although every cell biologist knows the importance of selecting the right growth conditions and it is well known that the composition of growth medium may vary depending on a product brand or lot affecting many cellular processes, still those effects are poorly systematized. We addressed this issue by comparing the effect of 12 fetal bovine sera (FBS) and eight growth media from different brands on the morphological and functional parameters of five cell types: lung adenocarcinoma, neuroblastoma, glioblastoma, embryonic kidney, and colorectal cancer cells. Using high-throughput imaging, we compared cell proliferation; performed morphological profiling based on the imaging of 561,519 cells; measured extracellular regulated kinases (ERK1/2) activity, mitochondria potential, and lysosome accumulation; and compared cell sensitivity to drugs, response to EGF stimulation, and ability to differentiate.
View Article and Find Full Text PDFBackground: The global demographic situation has been significantly impacted by the COVID-19 pandemic. The objective of this study was to develop a model that predicts the risk of COVID-associated mortality using clinical and laboratory data collected within 72 h of hospital admission.
Materials And Methods: A total of 3024 subjects with PCR-confirmed COVID-19 were admitted to Almazov National Research Medical Center between May 2020 and August 2021.
Article Synopsis
- Imaging-based anticancer drug screening is gaining traction due to advancements in fluorescence microscopy and image processing, enabling detailed study of cell viability and proliferation.
- Despite the availability of large-scale datasets for cell viability, the integration of toxicology measurements from these screens with imaging data remains uncertain.
- This research shows that imaging methods yield high accuracy in assessing drug effects across various cancer cell lines and that selecting specific analysis metrics can improve consistency in results while highlighting the benefits of monitoring mitochondria and cell dynamics.
[Serum brain damage biomarkers as a diagnostic and prognostic tool in ischemic stroke].
Zh Nevrol Psikhiatr Im S S Korsakova
September 2023
Objective: To study serum quantities of neuron specific enolase (NSE), glial fibrillary acidic protein (GFAP) and NR2-antibodies (NR2-ab) in various cerebrovascular pathology and assess their value as a panel used as a diagnostic and predictive tool for stroke.
Material And Methods: NSE, GFAP and NR2-ab serum levels were measured twice for 84 patients with ischemic stroke (IS) and 8 patients with hemorrhagic stroke (HI), once for 8 patients with transient ischemic attack (TIA), 26 patients with chronic brain ischemia (CBI), 27 healthy volunteers (HV).
Results: NSE and GFAP levels were significantly higher in IS than in CBI and HV patients, and NR2-ab levels in IS were higher than in TIA and lower than in HV.