[Effect of ouabain, iodoacetamide, and pH on the human erythrocyte hemolysis induced by calcium ions in the presence of ionophore A23187].

The effect of inhibitors of Na+, K(+)-ATPase (ouabain) and glycolysis (iodacetamide) as well as pH on calcium ion-induced erythrocyte hemolysis in the presence of ionophore A23187 is first described. Hemoglobin release decreases under the influence of ouabain, iodacetamide, and low pH, which is commonly observed at low temperature and in the samples studied in spring and summer. Active hemoglobin release through defects of the erythrocyte membrane under the influence of the transmembrane electrical potential was proposed to mediate hemolysis.

Adaptive changes of membrane lipid composition in humans living in the north.

Some parameters of red cell membrane lipid composition as well as intensiveness of lipid peroxidation and activity of its regulatory factors were assessed in northern aborigines, newcomers and alcohol abusers. It is proposed that the increased lipid peroxidation is responsible for the cholesterol and monoenic fatty acid accumulation in membranes of all groups studied. The data obtained make it possible to consider the lipid peroxidation as a mechanism for adaptive membrane lipid modification in humans.

[Myocardial catecholamine levels in patients with congenital and acquired heart defects].

Catecholamine levels were determined in the myocardial slices from 66 patients with congenital and acquired heart diseases by the trihydroxyindole++ method on a Lumilan fluorescence spectrophotometer. The patients were found to have greatly varying epinephrine and norepinephrine levels. The accumulation of catecholamine reflected, to a certain extent, the occurrence of disorders in relation to the functional load, hyperfunctions of the myocardium.