Transient Expression of Zika NS2B Protein Antigen in and Use for Arboviruses Diagnosis.

Zika (ZIKV) and Dengue (DENV) viruses are clinically significant due to their severe neurological and hemorrhagic complications. Rapid diagnostics often rely on nonstructural proteins to generate specific antibodies. This study aimed to produce IgG antibodies from the recombinant ZIKV protein and plant-expressed NS2B protein for arbovirus detection in serum and urine samples.

A survey of lip lesions diagnosed in a single institution: A clinicopathological study.

Background: The aim of this study was to assess the clinicopathological features of lip lesions diagnosed in a single Oral Pathology service in Brazil.

Material And Methods: It was a cross-sectional study based on secondary data. Between 2000 and 2019, all lip lesions diagnosed in an Oral Pathology service in Brazil were analyzed.

High detection rate of parasitic load by qPCR targeting 18S rDNA in blood of patients with active leishmaniasis lesions.

This study aimed to standardize qPCR techniques using these molecular markers kDNA and 18S rDNA across three sample types: peripheral blood, guanidine-treated blood, and tissue. The secondary objective is to evaluate the performance of 18S rDNA target in samples from 46 patients with confirmed tegumentary leishmaniasis. After obtaining the standard curve from reference strains with Leishmania, qPCR curves were standardizations and the Cts results of the patient samples were described using abstract measures.

Integrative physiological, biochemical, and proteomic analysis of the leaves of two cotton genotypes under heat stress.

Cotton (Gossypium hirsutum L.), a crucial global fibre and oil seed crop faces diverse biotic and abiotic stresses. Among these, temperature stress strongly influences its growth, prompting adaptive physiological, biochemical, and molecular changes.

Factors associated with death among indigenous and non-indigenous pregnant and postpartum women hospitalized for COVID-19 in Brazil.

The present article aimed to analyze the association between sociodemographic and hospitalization characteristics with the outcome of indigenous and non-indigenous pregnant and postpartum women, as well as factors associated with deaths among indigenous women hospitalized for Severe Acute Respiratory Syndrome (SARS) due to COVID-19 in Brazil. This is a cross-sectional and analytical study, with secondary data of pregnant and postpartum women of reproductive age, classified into race/skin color (indigenous and non-indigenous), extracted from the Obstetric Observatory, which uses data from the Influenza Epidemiological Surveillance Information System. The outcome variables were analyzed using the chi-square test or Fisher's exact test, and logistic regression was performed for the factors associated with the death of indigenous people.