Comparative evaluation of ACetic - MEthanol high salt dissociation approach for single-cell transcriptomics of frozen human tissues.

Current dissociation methods for solid tissues in scRNA-seq studies do not guarantee intact single-cell isolation, especially for sensitive and complex human endocrine tissues. Most studies rely on enzymatic dissociation of fresh samples or nuclei isolation from frozen samples. Dissociating whole intact cells from fresh-frozen samples, commonly collected by biobanks, remains a challenge.

Chromosomal Abnormalities in Miscarriages and Maternal Age: New Insights from the Study of 7118 Cases.

Chromosomal abnormalities of the embryo are the most common cause of first-trimester pregnancy loss. In this single-center study, we assessed the frequency and the spectrum of chromosomal abnormalities in miscarriages for each year of maternal age from 23 to 44. Cytogenetic data were obtained by conventional karyotyping of 7118 miscarriages in women with naturally conceived pregnancies.

Multi-target neural network model of anxiolytic activity of chemical compounds using correlation convolution of multiple docking energy spectra.

Anxiety disorders are one of the most common mental health pathologies in the world. They require searc h and development of novel effective pharmacologically active substances. Thus, the development of new approaches to the search for anxiolytic substances by artificial intelligence methods is an important area of modern bioinformatics and pharmacology.

[Diagnosis of neuroinfections in children].

Presents an overview of domestic and foreign sources on the diagnosis of neuroinfections in children of different etiologies based on epidemiological, physical, cerebrospinal fluid, etiological, radiation and other methods. Their combination makes it possible to establish the syndrome of neuroinfection (meningitis, encephalitis, myelitis, polyradioloneuritis, etc.), the severity, the presence of complications, the nature of the course, etiology.