Implementing Music Medicine to Improve Pain Outcomes After Abdominal or Pelvic Surgery: A Quality Improvement Project.

Purpose: Define the impact of the implementation of evidence-based music medicine (MM) on pain management outcomes after abdominal and pelvic surgery when combined with usual care.

Design: This quality improvement project implemented MM in a hospital postanesthesia care unit using a pretest-posttest design. The Roy Adaptation Model and Kotter's Change theory guided all project phases.

Clathrin light chains are required for the gyrating-clathrin recycling pathway and thereby promote cell migration.

The clathrin light chain (CLC) subunits participate in several membrane traffic pathways involving both clathrin and actin, through binding the actin-organizing huntingtin-interacting proteins (Hip). However, CLCs are dispensable for clathrin-mediated endocytosis of many cargoes. Here we observe that CLC depletion affects cell migration through Hip binding and reduces surface expression of β1-integrin by interference with recycling following normal endocytosis of inactive β1-integrin.

Anisotropic nanocrystal arrays organized on protein lattices formed by recombinant clathrin fragments.

Recombinant clathrin protein fragments form assemblies that template gold nanocrystals in an array across the latticed surface. The nanocrystals exhibit unusual anisotropic morphologies with long range ordering, both of which are dependent upon the presence of a hexahistidine tag on the clathrin heavy chain fragments.

Clathrin promotes centrosome integrity in early mitosis through stabilization of centrosomal ch-TOG.

Clathrin depletion by ribonucleic acid interference (RNAi) impairs mitotic spindle stability and cytokinesis. Depletion of several clathrin-associated proteins affects centrosome integrity, suggesting a further cell cycle function for clathrin. In this paper, we report that RNAi depletion of CHC17 (clathrin heavy chain 17) clathrin, but not the CHC22 clathrin isoform, induced centrosome amplification and multipolar spindles.

Intracellular processing of riboflavin in human breast cancer cells.

A variety of polarized epithelial cells, such as human breast cancer (MCF-7), have mechanistically evolved the ability to adapt to the dynamic cellular environment and maintain homeostasis of an array of micronutrients which display conditional requirements. Active absorption mechanisms, including endocytosis, are able to control cell surface recognition and protein expression which are associated with a substance's intracellular processing and kinetics. Riboflavin (RF), or vitamin B2, has been recognized as an important factor in a multitude of terminal disease states, most notably in breast cancer, where its cellular absorption is significantly enhanced.