Publications by authors named "A G Brox"
The BonnMu resource is a transposon tagged mutant collection designed for functional genomics studies in maize. To expand this resource, we crossed an active Mutator (Mu) stock with dent (B73, Co125) and flint (DK105, EP1, and F7) germplasm, resulting in the generation of 8064 mutagenized BonnMu F-families. Sequencing of these Mu-tagged families revealed 425 924 presumptive heritable Mu insertions affecting 36 612 (83%) of the 44 303 high-confidence gene models of maize (B73v5).
View Article and Find Full Text PDFIdentification of Transposon Insertion Sites in Maize -Tagged Mutants Using Mu-Seq.
Cold Spring Harb Protoc
September 2024
() transposons facilitate untargeted insertional mutagenesis in maize by moving within the genome and disrupting genes. Such an approach has been used to generate collections such as the resource, a tagged maize population for functional genomics studies. Mutant-Seq (Mu-Seq) is a sequencing-based method for the high-throughput identification and mapping of insertion sites.
View Article and Find Full Text PDFArticle Synopsis
- The text discusses a collection of maize mutants created through transposon insertions, aimed at aiding functional genomics research.
- It outlines methodologies for both reverse genetics—where researchers identify and characterize mutations using existing genetic data and confirmation techniques—and forward genetics, which involves using advanced sequencing methods to find the genes responsible for observed traits.
- The overall goal is to establish connections between specific genetic mutations and their effects on observable characteristics in maize.
Article Synopsis
- Redox status, which helps control proteins, is influenced by two systems: glutathione (GSH) and glutaredoxin (GRX), along with thioredoxin (TRX).
- Scientists studied a specific protein (PpGRXC5) in moss to see how it affects other proteins when changes happen, like an increase in oxidation.
- Even though PpGRXC5 helps keep protein balance, plants without it still grew normally and handled stress fine, showing that this protein's role might be more about fine-tuning rather than being super essential.
The makeup of a new surgical bariatric team may be associated with a higher number of postoperative complications due to the learning curve. The aim of this study was to evaluate the outcomes during the learning curve of laparoscopic gastric bypass (LGBP) depending on surgeons' training. A systematic approach was used to review studies from the Pubmed, Embase (Ovid), Cancer Lit, Biomes Central via Scirus, Current Contens (ISI), and Web of Science (SCI) databases.
View Article and Find Full Text PDF