Publications by authors named "A Freis"
We aimed to determine whether a functional link with impact on female ovarian reserve exists between expression and expression ratios of AKT/mTOR signaling genes in human granulosa cells in vivo, as suggested from prior in vitro data. Three hundred and nine women, who were classified as normal (NOR; = 225) and poor (POR; = 84) responders based on their ovarian reserve, were recruited during stimulation for assisted reproductive techniques. Expressions of and of key genes of the AKT/mTOR and AKT/FOXO1/3 signaling pathways were comparatively analyzed in their granulosa cells.
View Article and Find Full Text PDFIn order to improve ART outcome, non-invasive embryo assessment is gaining more and more attention. Therefore, the aim of this study is to determine the consecutive implantation potential via the secretome between blastocysts with or without implantation and to analyse possible interactions between these differentially expressed proteins. In this prospective study, 69 spent culture media from blastocysts transferred at day 5 were collected from patients undergoing IVF/ICSI treatment in a single IVF centre between April 2015 and November 2018 after informed consent and analysed individually.
View Article and Find Full Text PDFArticle Synopsis
- Endometriosis (EM) affects 10-15% of women of reproductive age, but its molecular mechanisms are not fully understood, and diagnosis typically relies on laparoscopic surgery due to a lack of clinical biomarkers.
- Researchers analyzed endometrial tissue samples from 58 women to investigate gene and protein expression of specific molecules (GRN, NOTCH3, FN1, PINK1) associated with EM.
- Findings indicate that GRN and FN1 may serve as potential clinical biomarkers for detecting endometriosis, with variations in expression correlating to disease severity, but further research with a larger sample size is required to validate these results.
The impact of controlled ovarian stimulation (COS) during medically assisted reproduction (MAR) on human embryogenesis is still unclear. Therefore, we investigated if early embryonic development is affected by the type of gonadotropin-releasing hormone (GnRH) analog used to prevent a premature LH surge. We compared embryo morphology and morphokinetics between GnRH agonist and antagonist cycles, both involving human chorionic gonadotropin (hCG)-trigger.
View Article and Find Full Text PDFMotherhood after cancer: fertility and utilisation of fertility-preservation methods.
Arch Gynecol Obstet
June 2020
Purpose: Due to modern and individualised treatments, women at reproductive age have a high survival rate after cancer therapy. What are pregnancy and birth rates of women after cancer and how often do they use cryopreserved ovarian tissue or gametes?
Methods: From 2007 to 2015, 162 women aged 26.7 ± 6.