Publications by authors named "A Filby"
Current rheumatoid arthritis (RA) treatments do not restore immune tolerance. Investigating dendritic cell (DC) populations in human synovial tissue (ST) may reveal pathways to reinstate tolerance in RA. Using single-cell and spatial transcriptomics of ST biopsies, as well as co-culture systems, we identified condition- and niche-specific DC clusters with distinct functions.
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- The study investigates the immune mechanisms behind chronic lung allograft dysfunction (CLAD), which hampers long-term survival after lung transplants, using advanced tissue imaging techniques.
- Researchers analyzed lung tissue from 23 transplant recipients, identifying differences in immune cell populations associated with CLAD versus non-CLAD conditions.
- Key findings show that specific immune cells, like cytotoxic T cells and γδ T cells, expand in CLAD, highlighting unique characteristics in different CLAD phenotypes and offering new insights into how fibrosis progresses in these conditions.*
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- The study created a comprehensive reference atlas of human prenatal skin (7-17 weeks post-conception) using advanced techniques like single-cell and spatial transcriptomics to explore the roles of immune cells, specifically macrophages, in skin development.
- It was found that interactions between immune and non-immune cells are essential for key processes in skin development, such as hair follicle formation, scarless wound healing, and blood vessel growth.
- Additionally, while a skin organoid model mimicked certain features of prenatal skin, it lacked immune cells and showed limited blood vessel diversity, highlighting the important roles of macrophages and their derived factors in skin morphology and development.
Imaging flow cytometry (IFC) provides single-cell imaging data at a high acquisition rate. It is increasingly used in image-based profiling experiments consisting of hundreds of thousands of multi-channel images of cells. Currently available software solutions for processing microscopy data can provide good results in downstream analysis, but are limited in efficiency and scalability, and often ill-adapted to IFC data.
View Article and Find Full Text PDFLeukemia niche impacts quiescence; however, culturing patient-derived samples ex vivo is technically challenging. Here, we present a protocol for in vitro co-culture of patient-derived xenograft acute lymphoblastic leukemia (PDX-ALL) cells with human mesenchymal stem cells (MSCs). We describe steps for labeling PDX-ALL cells with CellTrace Violet dye to demonstrate MSC-primed PDX-ALL cycling.
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