Role of N-glycosylation of 66 and 69 kDa glycoproteins in wall formation during pollen tube growth in vitro.

Two abundant cell wall glycoproteins (66 and 69 kDa) accumulate during growth in pollen tubes of tobacco. Glycosylation of the proteins was experimentally modified by application of the specific inhibitors tunicamycin and castanospermine to in vitro cultured pollen. Newly synthesized proteins were labeled with a 14C-amino acid mixture supplied to the medium.