Sequencing of the VP1-2A Genome Segment for Hepatitis A Subtyping.

Unlabelled: was to improve the VP1-2A sequencing method and facilitate the hepatitis A virus genotyping technique.

Materials And Methods: Nucleic acids were extracted by using commercial kits for nucleic acid extraction and reverse transcription in a manual and automatic version. Primer sequences were selected using the DNASTAR software.

[Data on the epidemiology of rotavirus infection].

The findings of epidemiological study of rotavirus infections among children in Gorki over the period of 1981-1985 are analyzed. The age of the patients is specified, as are the disease seasonal patterns and the incidence rates of its manifest and asymptomatic forms, and of carriership cases among healthy children in different foci of this infection.

[Assessment of the separate elements of an epidemiological overview of poliomyelitis today].

The materials on the epidemiological supervision of poliomyelitis in a large city, carried out in 1972-1981, are presented. Two periods differing in morbidity rate, in the level of population immunity to poliomyelitis and in the activity of the poliovirus circulation are defined. The supervision of poliomyelitis is shown to be capable of influencing the course of the epidemic process of this infection provided that the individual elements of such supervision are correctly evaluated in different epidemic situations.

[Rapid identification of enteroviruses by countercurrent immunoelectrophoresis].

The results of identification of 115 enterovirus strains by means of counter immunoelectrophoresis and the neutralization tests in cell culture are presented. Even at a high rate of agreement of the two methods, counter immuno-electrophoresis was shown to be more rapid, economic, less labour-consuming but requiring concentrated viral antigens.