Identifying the core genome of the nucleus-forming bacteriophage family and characterization of Erwinia phage RAY.

We recently discovered that some bacteriophages establish a nucleus-like replication compartment (phage nucleus), but the core genes that define nucleus-based phage replication and their phylogenetic distribution were still to be determined. Here, we show that phages encoding the major phage nucleus protein chimallin share 72 conserved genes encoded within seven gene blocks. Of these, 21 core genes are unique to nucleus-forming phage, and all but one of these genes encode proteins of unknown function.

Identifying the core genome of the nucleus-forming bacteriophage family and characterization of phage RAY.

We recently discovered that some bacteriophages establish a nucleus-like replication compartment (phage nucleus), but the core genes that define nucleus-based phage replication and their phylogenetic distribution were unknown. By studying phages that encode the major phage nucleus protein chimallin, including previously sequenced yet uncharacterized phages, we discovered that chimallin-encoding phages share a set of 72 highly conserved genes encoded within seven distinct gene blocks. Of these, 21 core genes are unique to this group, and all but one of these unique genes encode proteins of unknown function.

Pseudocell Tracer-A method for inferring dynamic trajectories using scRNAseq and its application to B cells undergoing immunoglobulin class switch recombination.

Single cell RNA sequencing (scRNAseq) can be used to infer a temporal ordering of cellular states. Current methods for the inference of cellular trajectories rely on unbiased dimensionality reduction techniques. However, such biologically agnostic ordering can prove difficult for modeling complex developmental or differentiation processes.

[The feasibility of using Quick Dementia Rating System in clinical practice: results of a multi-center prospective validation study].

Aim: To determine the sensitivity and specificity of the Quick Dementia Rating System (QDRS), a 10-item questionnaire for the assessment of cognitive, behavioral, and functional impairments, in the Russian population.

Material And Methods: The authors examined 2357 patients,1479 female (63%) and 878 male (37%), with subjective cognitive impairment (SCI), mild cognitive impairment (MCI) or dementia. Their average age was 73,1±9,7.

[Modern trends in epidemiology of dementia and management of patients with cognitive impairment].

Dementia is an important medical problem with the prevalence and significance growing year after year. Improvement of diagnostic methods facilitates early diagnosis and treatment. This year marks the 20th anniversary of memantine, one of the basic anti-dementia drugs, registration in Russia.