Luminescence enhancement by mixing carboxylate benzoate-pentafluorobenzoate ligands in polynuclear {EuZn} and {TbZn} complexes.

This study investigates mixed-carboxylate benzoate (bz)-pentafluorobenzoate (pfb) {EuZn} and {TbZn} compounds with 1,10-phenanthroline (phen) molecules. It is demonstrated that variation of the synthesis conditions yields mixed-carboxylate compounds with different compositions: [LnZn(phen)(bz)(pfb)] (Ln = Eu (1Eu), Tb (1Tb)) and [EuZn(phen)(bz)(pfb)]·4MeCN (2Eu). In these structures, bz and pfb anions occupy specific positions in various ratios.

A simple, highly sensitive and specific serological test for the detection of antibodies to Varicella-zoster virus ().

Introduction: Varicella-Zoster virus (VZV) is a highly contagious alpha-herpes virus. The diagnosis of chickenpox remains a difficult task especially in cases of breakthrough chickenpox, so the development of reliable laboratory tests is necessary. The simplest and most sensitive serological test for detecting antibodies in human and animal sera is the passive hemagglutination reaction (PHAR).

Infrared Spectral Patterns of Thyroglobulin Bearing Thyroiditogenic Epitopes.

Thyroglobulin is a major autoantigen to which autoimmune response, destroying the thyroid gland in Hashimoto's thyroiditis, is directed. To detect a pathological autoimmune response to thyroglobulin, as well as the successful induction of experimental autoimmune thyroiditis, thyroglobulin carrying thyroiditogenic epitopes is necessary. It is not known which features of thyroglobulin structure determine the presence of thyroiditogenic epitopes and can serve as markers of their presence.

Analysis of Toxicity of Recombinant Pneumolysin from Streptococcus pneumoniae.

We studied toxicity of recombinant Streptococcus pneumoniae pneumolysin protein in experiments on mice and its cytopathogenic effect on cultures of Vero green monkey kidney cells and human lung carcinoma A549 cells in vitro. In vivo and in vitro experiments proved the absence of compromised toxicity and direct cytopathogenic action of the recombinant protein.

Magnetic Nanoparticle Support with an Ultra-Thin Chitosan Layer Preserves the Catalytic Activity of the Immobilized Glucose Oxidase.

Here, we developed magnetically recoverable biocatalysts based on magnetite nanoparticles coated with an ultra-thin layer (about 0.9 nm) of chitosan (CS) ionically cross-linked by sodium tripolyphosphate (TPP). Excessive CS amounts were removed by multiple washings combined with magnetic separation.