Publications by authors named "A F Mulder"
Background: Video review is a feasible, commonly used learning tool, but current literature lacks a comprehensive review of its impact on learning in postgraduate medical education. This systematic review aims at examining the learning effect of video review of resident performance in clinical practice during postgraduate medical education.
Methods: A systematic literature search was conducted from May 2023 to July 2023 with an update on 12/12/2023.
Article Synopsis
- CD2, CD25, and CD30 expression in extracutaneous mast cells is a minor diagnostic criterion for systemic mastocytosis (SM), as categorized by the World Health Organization and International Consensus Classification.
- A study of 5,034 patients revealed that lower percentages of CD2, CD25, and/or CD30 in mast cells are seen in indolent SM compared to advanced forms like aggressive SM and mast cell leukemia.
- The absence of CD2 in mast cells is linked to significantly lower overall survival and indicates the potential for disease spread outside the bone marrow to organs like the spleen and liver.
Article Synopsis
- Whole genome sequencing (WGS) of Mycobacterium tuberculosis complex (MTBC) is greatly affected by the effectiveness of DNA extraction protocols, particularly the lysis step.
- In a study comparing four different DNA extraction methods, a bead beating protocol (protocol B) significantly increased DNA yield by 60-fold and improved sequencing success rates.
- Incorporating mechanical lysis not only enhances extraction from primary cultures but also enables earlier WGS from enriched cultures, ultimately reducing turnaround time while maintaining high-quality DNA needed for analysis.
Article Synopsis
- Giant cell arteritis (GCA) and polymyalgia rheumatica (PMR) are inflammatory diseases, and researchers are looking for biomarkers to identify active cases and those who may need alternative treatments to glucocorticoids.
- A study evaluated the presence of interferon type I (IFN-I) markers in GCA and PMR patients, including those not previously treated, but did not find significant differences in IFN-I scores between these groups and healthy controls.
- The results indicated that the IFN-I signature and serum markers CXCL10 and Galactin-9 were not elevated in patients with GCA or PMR, suggesting that these IFN-I markers may not be useful for diagnosing or guiding treatment
Defining what constitutes a healthy microbiome throughout our lives remains an ongoing challenge. Understanding to what extent host and environmental factors can influence it has been the primary motivation for large population studies worldwide. Here, we describe the fecal microbiome of 3,746 individuals (0-87 years of age) in a nationwide study in the Netherlands, in association with extensive questionnaires.
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