Comparison of multi-parallel qPCR and double-slide Kato-Katz for detection of soil-transmitted helminth infection among children in rural Bangladesh.

There is growing interest in local elimination of soil-transmitted helminth (STH) infection in endemic settings. In such settings, highly sensitive diagnostics are needed to detect STH infection. We compared double-slide Kato-Katz, the most commonly used copromicroscopic detection method, to multi-parallel quantitative polymerase chain reaction (qPCR) in 2,799 stool samples from children aged 2-12 years in a setting in rural Bangladesh with predominantly low STH infection intensity.

Transfer, transformation, and impacts of ceria nanomaterials in aquatic mesocosms simulating a pond ecosystem.

Mesocosms are an invaluable tool for addressing the complex issue of exposure during nanoecotoxicological testing. This experimental strategy was used to take into account parameters as the interactions between the NPs and naturally occurring (in)organic colloids (heteroaggregation), or the flux between compartments of the ecosystems (aqueous phase, sediments, biota) when assessing the impacts of CeO2 NPs in aquatic ecosystems. In this study, we determine the transfer, redox transformation, and impacts of 1 mg L(-1) of bare and citrate coated CeO2-NPs toward an ecologically relevant organism (snail, Planorbarius corneus) exposed 4 weeks in a complex experimental system mimicking a pond ecosystem.

Mobilization and engraftment of peripheral blood stem cells in healthy related donors >55 years old.

Background Aims: The increasing scarcity of young related donors has led to the use of older donors for related allogeneic hematopoietic stem cell transplantation (HSCT). This study analyzed the influence of age on the results of mobilization of peripheral blood stem cells (PBSCs) in healthy donors as well as on the engraftment and outcome of HSCT.

Methods: A retrospective analysis from a single center was performed comparing the results of PBSC mobilization from related healthy donors according to their age.

TM4SF20 ancestral deletion and susceptibility to a pediatric disorder of early language delay and cerebral white matter hyperintensities.

White matter hyperintensities (WMHs) of the brain are important markers of aging and small-vessel disease. WMHs are rare in healthy children and, when observed, often occur with comorbid neuroinflammatory or vasculitic processes. Here, we describe a complex 4 kb deletion in 2q36.

Predictive factors for poor peripheral blood stem cell mobilization and peak CD34(+) cell count to guide pre-emptive or immediate rescue mobilization.

Background Aims: Failure in mobilization of peripheral blood (PB) stem cells is a frequent reason for not performing hematopoietic stem cell transplantation (HSCT). Early identification of poor mobilizers could avoid repeated attempts at mobilization, with the administration of pre-emptive rescue mobilization.

Methods: Data from the first mobilization schedule of 397 patients referred consecutively for autologous HSCT between 2000 and 2010 were collected.