Publications by authors named "A Ermisch"
Regulation of Oocyte mRNA Metabolism: A Key Determinant of Oocyte Developmental Competence.
Adv Anat Embryol Cell Biol
July 2024
The regulation of mRNA transcription and translation is uncoupled during oogenesis. The reason for this uncoupling is two-fold. Chromatin is only accessible to the transcriptional machinery during the growth phase as it condenses prior to resumption of meiosis to ensure faithful segregation of chromosomes during meiotic maturation.
View Article and Find Full Text PDFThe refinement of embryo culture media is essential in improving embryo viability and in vitro production efficiency. Our previous work demonstrated that the nutrients (carbohydrates, amino acids, and vitamins) in traditional culture media far exceed the need for an embryo and producing developmentally competent embryos in a reduced nutrient environment is feasible. Here, we aim to evaluate the impact of exogenous lipid and L-carnitine supplementation on bovine blastocyst development and refine our RN condition further.
View Article and Find Full Text PDFObese women are subfertile and have reduced assisted reproduction success, which may be due to reduced oocyte competence. We hypothesize that consumption of a high-fat/high-sugar diet induces ovarian inflammation, which is a primary contributor to decreased oocyte quality and pre-implantation embryo development. To test this hypothesis, C57BL/6 (B6) mice with a normal inflammatory response and C3H/HeJ (C3H) mice with a dampened inflammatory response due to dysfunctional Toll-like receptor 4 were fed either normal chow or high-fat/high-sugar diet.
View Article and Find Full Text PDFObjective: To characterize fatty acid (FA) profile of commercially available albumin products and determine their effect on embryonic development.
Design: Research study.
Setting: Private research facility.
Article Synopsis
- Recent studies show that bovine embryos use only a fraction of available nutrients in their culture medium, prompting research on how lower nutrient levels affect their development.
- In experiments, embryos were cultured with significantly reduced nutrient concentrations (75% to as low as 6.25%) and results revealed that while reduced nutrients initially affected cell allocation, blastocyst formation and hatching were only inhibited at extremely low levels (6.25%).
- The reduction in nutrients activated metabolic pathways (AMPK) and inhibited others (mTOR), indicating a shift in how embryos manage energy and nutrients, highlighting the need for optimized culture media for better embryo development.