Publications by authors named "A Elsholz"
Genetic tools form the basis for the study of molecular mechanisms. Despite many recent advances in the field of genetic engineering in bacteria, genetic toolsets remain scarce for non-model organisms, such as the obligatory human pathogen To overcome this limitation and enable the straightforward investigation of gene functions in , we have developed a comprehensive genetic toolset. By adapting and combining different tools previously applied in other Gram-positive bacteria, we have created new replicative and integrative plasmids for gene expression and genetic manipulation, constitutive and inducible promoters as well as fluorescence reporters for .
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- Bacterial DNA replication begins at the origin of replication, where DnaA boxes allow the master initiator protein DnaA to bind and control the process.
- The study used CRISPR interference to halt replication by targeting specific DnaA boxes, leading to continued cell growth and translation without triggering stress responses.
- Understanding non-replicating states in bacteria can help uncover mechanisms of antibiotic tolerance, dormancy, and adaptability to harsh environments.
The cellular proteome comprises all proteins expressed at a given time and defines an organism's phenotype under specific growth conditions. The proteome is shaped and remodeled by both protein synthesis and protein degradation. Here, we developed a new method which combines metabolic and chemical isobaric peptide labeling to simultaneously determine the time-resolved protein decay and synthesis in an intracellular human pathogen.
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- The Bacillus subtilis spore coat consists of over 80 proteins organized into distinct layers, with CotB being a major component of the outer coat.
- CotG, another coat protein, interacts with CotB and enhances its phosphorylation by the kinase CotH, which is essential for proper outer coat structure.
- Mutations affecting CotH or CotG disrupt the formation of striations in the outer coat, highlighting the importance of this phosphorylation process for spore surface layer development.