Novel silver nanoparticle-based biomaterials for combating biofilms.

Background: is a significant nosocomial pathogen that has developed resistance to multiple antibiotics, often forming biofilms that enhance its virulence. This study investigated the efficacy of a novel nanoformulation, AgNPs@chitosan-NaF, in combating biofilms.

Methods: Antimicrobial susceptibility testing was performed to assess the antibiotic resistance profile of isolates.

Vancomycin-resistant Staphylococcus aureus endangers Egyptian dairy herds.

The emergence of pandrug-resistant (PDR) and extensive drug-resistant (XDR) methicillin-resistant and vancomycin-resistant Staphylococcus aureus (MRSA and VRSA) isolates from bovine milk samples along with biofilm formation ability and harboring various virulence genes complicates the treatment of bovine mastitis and highlights the serious threat to public health. This study investigated for the first time the frequency, antimicrobial resistance profiles, biofilm-forming ability, virulence factors, spa and staphylococcal cassette chromosome mec (SCCmec) types of MRSA and VRSA isolated from clinical and subclinical bovine mastitis in Egypt. A total of 808 milk samples were collected from each quarter of 202 dairy animals, including 31 buffaloes and 171 cattle.

Biocontrol of virulent Listeria monocytogenes using green carboxylated cellulose nanocrystals-silver nano-biohybrids.

L. monocytogenes is a Gram-positive bacterial pathogen, known to cause food poisoning and systemic disease, specifically listeriosis. This species has shown resistance to many commonly used antibiotics, making the search for new alternative therapies is a pressing matter.

Natural Inhibitors of MDR Efflux Pumps AcrAB and AcrD: An Integrated In Silico, Molecular, and In Vitro Investigation.

Multidrug-resistant (MDR) remains a significant global health threat. This study aimed to explore the potential of essential oil components as novel inhibitors of the MDR efflux pumps AcrAB and AcrD. isolates were characterized for serotype, antibiotic resistance, and efflux pump activity.

Deciphering the potential of (DC.) Stapf as an anti-obesity agent: phytochemical profiling, evaluations and molecular docking studies.

Based on its anti-inflammatory and antioxidant properties, (DC) Stapf is commonly used in traditional and modern medicine to cure different diseases. The present study investigates the potential of organic extract as an anti-obesity drug in a HCHFD (high-carbohydrate, high-fat diet) model for obese rats. Its negative hypolipidemic effect has been confirmed through biochemical and histological methods.