[Induction of the endoplasmic reticulum stress in conditions of acid-base imbalance in human cells of T-lymphoblastic leukemia].

Endoplasmic reticulum stress - typical molecular pathophysiological process that underlies many cardiovascular, endocrine and other diseases. Violations of the protein conformational maturation processes in the endoplasmic reticulum can cause proteotoxic stress. Compensatory mechanisms are activated in response to ER stress include increased expression of enzymes involved in the formation of disulfide bonds in proteins.

[Deposition of von Willebrand factor in human endothelial cells HUVEC in the endoplasmic reticulum stress induced by an excess of homocysteine in vitro].

Von Willebrand factor (vWF) is an adhesive glycoprotein synthesized and secreted by endothelial cells and megakaryocytes. Violation of vWF secretion by endothelial cells is a characteristic feature of endothelial dysfunction in hyperhomocysteinemia. In our study we examined to clarify the concentration-dependent effect of homocysteine (Hcy) on the expression of vWF.

[Multiple medication resistance of apoptosis-resistant tumoral cells].

A4 clone cells, received by CD95-mediated selection from the parental line of Jurkat T-lymphoblast human leukosis, lost their ability of apoptosis as a result of programmed cell death mechanism breakdown. The complex of their acquired phenotypic properties meets tumor progression criteria: oxidative stress resistance, active immune suppression, and low requirement for growth factors. The loss of A4 cell ability of apoptosis is accompanied by acquisition of the phenotype of multiple medication resistance to a wide spectrum of antineoplastic chemotherapeutic drugs and cytotoxins.

[Effect of arsenic on bacterial growth and plasma membrane atpase activity].

The effects of arsenic in the forms of arsenite and arsenate on bacterial growth and plasma membranes ATPase activity of was studied. Correlation of The rate of ATP hydrolysis was found to be correlated with bacterial resistance to toxic arsenic ions. Detoxification of arsenate by resistant cultures of bacteria was suggested to be related with an increase in bacterial ATPase activity and the degree of ATPase mobilization.

[Apoptosis and proliferative activity of bone marrow cells in patients with aplastic syndromes as evidenced by trephine biopsy].

Aim: To clarify the effect of cyclosporin A (CSA) on the apoptosis and proliferative activity of bone marrow cells in patients with aplastic syndromes by trepanobiopsy evidence.

Materials And Methods: The TUNEL immunohistochemical assay was used to study apoptosis of bone marrow cells in the histological specimens from 24 patients: 8 with refractory anemia (RA), 8 with acute small-proportion leukemias [RA with excessive blasts (RAEB) + RAEB in transformation (RAEBt)], 3 with acute non-lymphoblastic leukemia (ANLL), 5 with lymphogranulomatosis (LGM). Control apoptosis examination was made in 10 patients treated with CSA.