[The awareness of primary health care physicians about methods of evaluation of risks of stroke].

The assessment of prevalence of acute cerebral circulation disorders in persons with arterial hypertension, together with study of awareness of primary health care physicians about methods of assessing risk of stroke, allows to judge effectiveness of measures preventing acute cerebral circulation disorders and to outline further ways preventing cerebrovascular complications of hypertension.The purpose of study was to investigate morbidity of acute cerebral circulation disorders and awareness of primary care physicians about clinical and instrumental methods assessing risk of stroke in persons with arterial hypertension.Materials and methods included official statistical materials on morbidity of intracerebral bleeding and cerebral infarction in population of Russia, the Chelyabinsk Oblast in 2008-2020, totals of survey of internists and emergency physicians in six regions of Russia.

Small-molecule inhibitor of OGG1 suppresses proinflammatory gene expression and inflammation.

The onset of inflammation is associated with reactive oxygen species and oxidative damage to macromolecules like 7,8-dihydro-8-oxoguanine (8-oxoG) in DNA. Because 8-oxoguanine DNA glycosylase 1 (OGG1) binds 8-oxoG and because -deficient mice are resistant to acute and systemic inflammation, we hypothesized that OGG1 inhibition may represent a strategy for the prevention and treatment of inflammation. We developed TH5487, a selective active-site inhibitor of OGG1, which hampers OGG1 binding to and repair of 8-oxoG and which is well tolerated by mice.

[Diagnosis, Prognosis, Monitoring of Cardiac Complications of Arterial Hypertension. Hypertensive Heart Disease].

Aim: of the study was further investigation of diagnostics and management of cardiac complications of essential hypertension (EH).

Methods: Retrospective analysis of annual reports of cardiological departments for 10 year period was carried out. Internists (n=330) from 6 regions of Russia participated in the study.

Validation and development of MTH1 inhibitors for treatment of cancer.

Background: Previously, we showed cancer cells rely on the MTH1 protein to prevent incorporation of otherwise deadly oxidised nucleotides into DNA and we developed MTH1 inhibitors which selectively kill cancer cells. Recently, several new and potent inhibitors of MTH1 were demonstrated to be non-toxic to cancer cells, challenging the utility of MTH1 inhibition as a target for cancer treatment.

Material And Methods: Human cancer cell lines were exposed in vitro to MTH1 inhibitors or depleted of MTH1 by siRNA or shRNA.

Application of amide hydrogen/deuterium exchange mass spectrometry for epitope mapping in human cystatin C.

Human cystatin C (hCC) is a small cysteine protease inhibitor whose oligomerization by propagated domain swapping is linked to certain neurological disorders. One of the ways to prevent hCC dimerization and fibrillogenesis is to enable its interaction with a proper antibody. Herein, the sites of interaction of hCC with dimer-preventing mouse monoclonal anti-hCC antibodies Cyst28 are studied and compared with the binding sites found for mAb Cyst10 that has almost no effect on hCC dimerization.