Inferior Vena Cava Collapsibility Index and Central Venous Pressure for Fluid Assessment in the Critically Ill Patient.

Objective: To determine the correlation of sonographic evaluation of inferior vena cava diameter and its collapsibility index with central venous pressure in both spontaneously breathing and mechanically ventilated patients in surgical ICU.    Study Design: Cross-sectional study.

Place And Duration Of Study: Surgical ICU, Lahore General Hospital from November 2020 to May 2021.

Actomyosin and vimentin cytoskeletal networks regulate nuclear shape, mechanics and chromatin organization.

The regulation of nuclear state by the cytoskeleton is an important part of cellular function. Actomyosin stress fibres, microtubules and intermediate filaments have distinct and complementary roles in integrating the nucleus into its environment and influencing its mechanical state. However, the interconnectedness of cytoskeletal networks makes it difficult to dissect their individual effects on the nucleus.

Conformation of the human immunoglobulin G2 hinge imparts superagonistic properties to immunostimulatory anticancer antibodies.

Monoclonal antibody (mAb) drugs that stimulate antitumor immunity are transforming cancer treatment but require optimization for maximum clinical impact. Here, we show that, unlike other immunoglobulin isotypes, human IgG2 (h2) imparts FcγR-independent agonistic activity to immune-stimulatory mAbs such as anti-CD40, -4-1BB, and -CD28. Activity is provided by a subfraction of h2, h2B, that is structurally constrained due its unique arrangement of hinge region disulfide bonds.