In vitro NIH3T3 mouse embryonic fibroblast cell model does not predict AAV2 or AAVdj-mediated cell transformation.

One of the potential risk factors of recombinant adeno-associated virus (rAAV)-based gene therapy is insertional mutagenesis, which has been associated with the development of hepatocellular carcinoma (HCC) in rAAV-treated neonatal mice. The objective of this study was to investigate if well-established in vitro cell transformation assays (CTA) in mouse cell lines can detect AAV2 or AAVdj-mediated cell transformation. Since AAV integration at the Rian locus in neonatal mice has been implicated in AAV-mediated HCC, an rAAV vector specifically targeting the mouse Rian locus and an additional rAAV vector previously shown to cause HCC in neonatal mice were both tested for the induction of cell transformation in NIH3T3 cells.

Comparison of the Cytotoxic Mechanisms of Different Garlic ( L.) Cultivars with the Crucial Involvement of Peroxisome Proliferator-Activated Receptor Gamma.

Garlic ( L.) is one of the oldest known useful plants, valued for thousands of years. This plant contains many biologically active compounds, including polyphenols, sterols, cysteine-sulfoxides, carbohydrates, proteins, and amino acids.

The Combination of Resveratrol and Conjugated Linoleic Acid Dienes Enhances the Individual Effects of These Molecules on De Novo Fatty Acid Biosynthesis in 3T3-L1 Adipocytes.

Consuming food containing ingredients with a documented impact on lipid metabolism can help fight overweight and obesity. The simplest way to reduce the level of fatty acids is to block their synthesis or increase the rate of their degradation. This study aimed to determine the effect of resveratrol, , conjugated linoleic acid (CLA), , CLA, and various variants of their combinations on de novo fatty acid biosynthesis in 3T3-L1 adipocytes.

A Squaramide-Crown Ether-Based Receptor and Polymer for Enhanced Lithium Chloride Extraction.

A non-mulitmacrocyclic ion pair receptor with the ability to cooperatively bind lithium cations and halide anions is presented. These receptors feature a squaramide function for anion binding and are directly linked to a benzo-4-crown-12 unit to achieve selectivity toward lithium salts. Ion pair receptors and demonstrated stronger anion binding in the presence of cobound cations, while anion receptor lacks this property.