Different adhesion types and active sensitivity of platelet subpopulations.

Two different adhesion patterns of blood platelets with different sensitivity to adenosine were demonstrated by means of reflection contrast microscopy with consecutive image analysis and cell affinity chromatography. High-performance liquid chromatography revealed that thrombin-induced serotonin release of adenosine-sensitive platelets was lower than that of adenosine-resistant cells. Our results indicate platelet heterogeneity and suggest that the platelets with lower adenosine sensitivity may be actively involved in the early interaction between platelets and injured endothelium.

[A rapid method of isolation of platelet membranes for radioligand and enzymatic assays].

A simple and rapid procedure for isolation of the total platelet membrane fraction by chromatography on Sepharose CL 6B has been developed. This method allows a rapid (25-30 min) one-step separation of the membrane fraction on 26 x 150 mm columns with a 20-21 mg recovery. The high degree of purity of membrane preparations was confirmed by a radioligand assay using [3H]adenosine and L-[3H]glutamic acid.

[Changes in the activity of lactate dehydrogenase isoformes in mouse rhabdomyosarcoma under different proliferation conditions].

The activity of lactate dehydrogenase (LDH) isoenzymes was studied in clones of rhabdomyosarcoma (RMS) MX-53, being transplanted on mice of CC57W line. Clones obtained in lungs after the intravenous injection of a tumor cell suspension were transplanted into subcutaneous connective tissue (SCT) and eye anterior chamber (EAC). A study of LDH isoenzymes spectrum in transplants and native muscle tissues has been made using polyacrylamide gel electrophoresis and densitometry.