[Comparative study of the effect of paracetamol, phenacetin and amidopyrine on mitochondrial function in isolated rat hepatocytes].

Effects of amidopyrine, phenacetin and paracetamol on the viability and energetic state of isolated rat hepatocytes were compared. During incubation in minimal salt solution all drugs in concentrations above 1 mM in dose-dependent manner decreased the viability of hepatocyte suspension assessed by trypan blue dye inclusion and inhibited the ATP synthesis and the respiratory activity. Cytotoxic effect of chemicals decrease in the order: amidopyrine-->phenacetin-->paracetamol.

[The effect of paracetamol and its combinations with acetylsalicylic and ascorbic acids on lipid peroxidation processes in the rat liver].

Paracetamolum (PC) after i.p. administration to rats at the therapeutic (100.

[Isolation of rat hepatocytes by a nonenzymatic method: detoxifying and respiratory activity].

A procedure for isolation of rat hepatocytes involving perfusion by EDTA, mechanical disaggregation of the liver and separation of intact cells from damaged ones by low-velocity centrifugation in isotonic sucrose media is described. A layer of a darker colour formed after separation contains hepatocytes with native plasma membrane characterized by respiratory activity and rate of biotransformation being close to the values obtained with cells isolated with the application of collagenase.