[Characterization, identification, and screening for tetrodotoxin production by bacteria associated with the Cephalotrix simula (Ivata, 1952) proboscis worm].

The taxonomic composition of bacteria associated with the Cephalotrix simula proboscis worm was studied and screening of the tetrodotoxin (TTX)-producing bacteria was carried out using confocal laser scanning microscopy and polyclonal antibodies. Bacterial isolates were identified using the 16S rRNA gene sequencing and phenotypic characteristics. A Bacillus species was found to be responsible for tetrodotoxin production in C.

On the colour types in Lycodes nakamurae (Tanaka, 1914) and species composition of the subgenus Furcimanus (Perciformes: Zoarcidae: Lycodes) in the sea of Japan.

Two colour types were revealed in a zoarcid fish of the subgenus Furcimanus, genus Lycodes, in the Sea of Japan. A comparison of morphometric, meristic and genetic characters in dark coloured and light coloured individuals suggests that the two colour morphs represent a single species, determined to be Lycodes nakamurae (Tanaka, 1914). Variability in colouration within L.

[Intraspecific polymorphism of mtDNA in Sakhalin taimen Parahucho perryi].

The intraspecific polymorphism of Sakhalin taimen Parahucho perryi (Brevoort, 1856), Salmonidae, was assessed via the RFLP analysis of mitochondrial DNA fragments that contain Cytb, the control region (D-loop), and the genes for the NADH dehydrogenase subunits 1-4, as well as the sequencing of the mitochondrial DNA COI gene. The statistically significant differentiation of the populations from the mainland coast of the Sea of Japan and the eastern and western coasts of Sakhalin Island was demonstrated. The higher level of haplotype diversity in Sakhalin populations compared to Primorye populations and the structure of haplotype genealogies suggested that all populations of Parahucho perryi originated from a common ancestor, which probably lived on the western coast of the Sakhalin Island.

[Analysis of the mitochondrial DNA variation in pond smelt Hypomesus olidus (Osmeridae)].

Pond smelt Hypomesus olidus (Pallas, 1814), one of the five species of the genus Hypomesus, family Osmeridae, was examined for intraspecific variation of the mitochondrial DNA cytb (1062 bp) and COI (567 bp) genes. Among the ten single substitutions discovered, only one, leading to the substitution of isoleucine by valine, was nonsynonymous, while the remaining substitutions were synonymous. The degree of genetic divergence among pooled nucleotide sequences in H.

[Genetic divergence and relationships among smelts of the genus Osmerus from the Russian waters].

Smelts of the genus Osmerus, O. eperlanus and O. mordax dentes, inhabiting the basins of the Atlantic, Arctic, and Pacific oceans were investigated using RFLP analysis of the mitochondrial DNA segments A8/A6/COIII/ND3 and ND3/ND4, and sequencing of the cytb and COI genes (mtDNA), and intron 1 of the rpS7 gene (nDNA).