Publications by authors named "A D Jeyasekharan"
Confocal microscopy has evolved to be a widely adopted imaging technique in molecular biology and is frequently utilized to achieve accurate subcellular localization of proteins. Applying colocalization analysis on image z-stacks obtained from confocal fluorescence microscopes is a dependable method of revealing the relationship between different molecules. In addition, despite the established advantages and growing adoption of 3D visualization software in various microscopy research domains, there have been few systems that can support colocalization analysis within a user-specified region of interest (ROI).
View Article and Find Full Text PDFDNA replication stress (RS) is a widespread phenomenon in carcinogenesis, causing genomic instability and extensive chromatin alterations. DNA damage leads to activation of innate immune signaling, but little is known about transcriptional regulators mediating such signaling upon RS. Using a chemical screen, we identified protein arginine methyltransferase 5 (PRMT5) as a key mediator of RS-dependent induction of interferon-stimulated genes (ISGs).
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- The study investigates the distinct regions of the germinal center (GC)—the dark zone (DZ) and light zone (LZ)—which are crucial for B-cell expansion and antibody maturation, yet lack a clear understanding of their immune composition differences.
- Researchers discovered specific DNA damage responses and chromatin features that explain why T-cells are excluded from the DZ region, providing insights into its immune-repulsive characteristics.
- The findings highlight the role of the ATR kinase in regulating responses in the DZ, suggesting that targeting ATR could enhance immunotherapy effectiveness for aggressive types of lymphoma like Diffuse Large B-cell Lymphomas (DLBCL).
Macrophages are abundant immune cells in the microenvironment of diffuse large B-cell lymphoma (DLBCL). Macrophage estimation by immunohistochemistry shows varying prognostic significance across studies in DLBCL, and does not provide a comprehensive analysis of macrophage subtypes. Here, using digital spatial profiling with whole transcriptome analysis of CD68+ cells, we characterize macrophages in distinct spatial niches of reactive lymphoid tissues (RLTs) and DLBCL.
View Article and Find Full Text PDFDeregulation of the DNA damage response (DDR) plays a critical role in the pathogenesis and progression of many cancers. The dependency of certain cancers on DDR pathways has enabled exploitation of such through synthetically lethal relationships e.g.
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