5' Transgenes drive leaky expression of 3' transgenes in Cre-inducible bi-cistronic vectors.

Molecular cloning techniques enabling contemporaneous expression of two or more protein-coding sequences provide an invaluable tool for understanding the molecular regulation of cellular functions. The Cre-lox system is used for inducing the expression of recombinant proteins encoded within a bi-/poly-cistronic cassette. However, leak expression of transgenes is often observed in the absence of Cre recombinase activity, compromising the utility of this approach.

Chinese FDI outflows and host country environment.

This study provides first evidence on the effects of Chinese FDI (Foreign Direct Investment) outflows on host country environments. The study draws upon a comprehensive dataset covering aggregate Chinese FDI outflows and sector specific data into 65 host nations over the 2007-2019 period. Employing a STIRPAT (Stochastic Regression on Population, Affluence and Technology) model and several different techniques including DID (Difference-in-Difference), pooled OLS (Ordinary Least Squares), quantile regression, IV (Instrumental Variable) estimation, threshold and Tobit regression, the findings suggest that Chinese FDI leads to an increase in host country CO2 (Carbon Dioxide) emissions, aligning with the pollution haven hypothesis at the aggregate level.

Choice Modeling for the Commercial Cultivation of Underutilized Aromatic Plants for Producing Mosquito Repellents: Targeting Rural Sector Income Generation.

Tropical countries face considerable economic losses due to mosquito-borne diseases which can be effectively combatted using plant-based mosquito repellents. Therefore, using a questionnaire survey, we selected the 25 top-ranked common but underutilized aromatic plants with mosquito repellent ability in Sri Lanka to investigate the rural sector's willingness to cultivate and supply them. , , , , , and were the common species thus identified.

Pharmacological Evidence Suggests That Slo3 Channel Is the Principal K Channel in Boar Spermatozoa.

Sperm ion channels are associated with the quality and type of flagellar movement, and their differential regulation is crucial for sperm function during specific phases. The principal potassium ion channel is responsible for the majority of K ion flux, resulting in membrane hyperpolarization, and is essential for sperm capacitation-related signaling pathways. The molecular identity of the principal K channel varies greatly between different species, and there is a lack of information about boar K channels.

Hexane fraction of thunb. Seed extract enhances boar sperm motility via CatSper ion channel.

Introduction: Mammalian sperm motility is facilitated by flagellar beating, which depends on active ion movement through ion channels and their regulation. Thunb., also known as oriental bush cherry, is a widely used traditional medicinal plant.