Molecular identity crisis: environmental DNA metabarcoding meets traditional taxonomy-assessing biodiversity and freshwater mussel populations (Unionidae) in Alabama.

The use of environmental DNA (eDNA) to assess aquatic biodiversity is a growing field with great potential for monitoring and managing threatened species, like freshwater mussel (Unionidae) populations. Freshwater mussels are globally imperiled and serve essential roles in aquatic systems as a food source and as a natural water filter making their management essential for ecosystem health. Unfortunately, mussel populations are often understudied, and challenges exist to accurately and efficiently describe the full suite of species present.

Detection and accumulation of environmentally-relevant glyphosate concentrations delivered via pulse- or continuous-delivery on passive samplers.

Glyphosate is the most commonly used herbicide globally, which has contributed to its ubiquitous presence in the environment. Glyphosate application rates and delivery to surface water vary with land use. Streams in urban and agricultural catchments can experience continuous delivery of low concentrations of glyphosate and aminomethylphosphonic acid (AMPA), while their presence in forest streams occurs as an episodic pulse following silvicultural application.

Land Use Overrides Stream Order and Season in Driving Dissolved Organic Matter Dynamics Throughout the Year in a River Network.

Anthropogenic land use has increased nutrient concentrations and altered dissolved organic matter (DOM) character and its bioavailability. Despite widespread recognition that DOM character and its reactivity can vary temporally, the relative influence of land use and stream order on DOM characteristics is poorly understood across seasons and the entire flow regime. We examined DOM character and 28-day bioavailable dissolved organic carbon (BDOC) across a river network to determine the relative roles of land use and stream order in driving variability in DOM character and bioavailability throughout the year.

Shifting stoichiometry: Long-term trends in stream-dissolved organic matter reveal altered C:N ratios due to history of atmospheric acid deposition.

Dissolved organic carbon (DOC) and nitrogen (DON) are important energy and nutrient sources for aquatic ecosystems. In many northern temperate, freshwater systems DOC has increased in the past 50 years. Less is known about how changes in DOC may vary across latitudes, and whether changes in DON track those of DOC.

Sensitivity and threshold dynamics of Pinus strobus and Quercus spp. in response to experimental and naturally occurring severe droughts.

Increased drought frequency and severity are a pervasive global threat, yet the capacity of mesic temperate forests to maintain resilience in response to drought remains poorly understood. We deployed a throughfall removal experiment to simulate a once in a century drought in New Hampshire, USA, which coupled with the region-wide 2016 drought, intensified moisture stress beyond that experienced in the lifetimes of our study trees. To assess the sensitivity and threshold dynamics of two dominant northeastern tree genera (Quercus and Pinus), we monitored sap flux density (Js), leaf water potential and gas exchange, growth and intrinsic water-use efficiency (iWUE) for one pretreatment year (2015) and two treatment years (2016-17).