RNA interference targeting nucleocapsid protein (C) inhibits classical swine fever virus replication in SK-6 cells.

The application of RNA interference (RNAi) strategy for controlling classical swine fever could become a promising alternative to the conventional eradication measures, as it was recently shown for foot-and-mouth disease (Chen et al., 2004), influenza (Ge et al., 2003), porcine reproductive and respiratory syndrome (He et al.

Ethnoveterinary study for antidermatophytic activity of Piper betle, Alpinia galanga and Allium ascalonicum extracts in vitro.

Crude ethanolic extracts of Piper betle leaves (Piperaceae), Alpinia galanga rhizomes (Zingiberaceae) and Allium ascalonicum bulbs (Liliaceae) were tested against selected zoonotic dermatophytes (Microsporum canis, Microsporum gypseum and Trichophyton mentagrophyte) and the yeast-like Candida albicans. A broth dilution method was employed to determine the inhibitory effect of the extracts and compared to those of ketoconazole and griseofulvin. All extracts suppressed the growth of the fungi in a concentration-dependent manner.

Application of latex beads agglutination test for the detection of the antibody against virus-infection-associated (VIA) antigen of foot-and-mouth disease (FMD) virus.

Latex beads agglutination (LA) for the detection of the antibody against virus infection-associated (VIA) antigen of foot-and-mouth disease (FMD) virus was estimated using experimentally infected animals. The VIA antibody titer by the LA test were compared with the neutralization titer and the titer by agarose gel diffusion (AGD) test, which has been used as a standard method for VIA antibody titration. The latex beads were coated with VIA antigen in carbonate buffer solution (0.

Estimation of 140S particles in foot-and-mouth disease virus (FMDV) vaccine by using the computer analyzing system.

The quantity of 140S particles in inactivated foot-and-mouth disease virus (FMDV) vaccine samples produced in Foot-and-Mouth Disease Vaccine Production Center (FMD Vaccine Production Center) in Thailand was estimated by the sucrose gradient ultracentrifugation and optical density analysis by using the computer applying system. The soft ware; Chromato Data System (CDS) (Nihon Chromato Works Co., Ltd.