RNA viruses have evolved elaborate strategies to protect their genomes, including 5' capping. However, until now no RNA 5' cap has been identified for hepatitis C virus (HCV), which causes chronic infection, liver cirrhosis and cancer. Here we demonstrate that the cellular metabolite flavin adenine dinucleotide (FAD) is used as a non-canonical initiating nucleotide by the viral RNA-dependent RNA polymerase, resulting in a 5'-FAD cap on the HCV RNA.
View Article and Find Full Text PDFSurvival of Ostertagia circumcincta eggs in faeces kept at 4 degrees C and of third stage larvae (L3) kept in distilled water at -50, -25, -10, 4, 16, 25 and 35 degrees C was studied. The effect of relative humidity (RH) of 95, 75, 50 and 30% at 16, 25 and 35 degrees C on the survival of L3 was also studied. The survival of eggs at 4 degrees C was high during the first week, followed by a gradual decline to 10% by Day 22 of storage.
View Article and Find Full Text PDFHatching of the eggs of Ostertagia circumcincta was studied by recovering them from faeces and incubating them in distilled water at temperatures of 4, 16, 25 and 35 degrees C. Hatching occurred at all the temperatures. The rate of hatching increased with the rise in temperature.
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