Publications by authors named "A Certoma"
Mitigation of African swine fever (ASF) virus in contaminated feed materials would assist control activities. Various finely-ground pig feed ingredients (5 cereals, 4 plant proteins, 2 animal proteins, 1 oil, 1 compound) were sprayed and mixed thoroughly with a buffered formic acid formulation (0, 1 or 2% vol/vol) to produce a consistent and durable level of formate (1% or 2%) with consistent acidification of cereal ingredients to less than pH 4. No such acidification was noted in other ingredients.
View Article and Find Full Text PDFHere, we report the complete genome sequence of the African swine fever virus (ASFV) isolate ASFV/Timor-Leste/2019/1, isolated from a domestic pig during the first outbreak of ASF in Timor-Leste in 2019. Using target enrichment short-read Illumina data combined with long-read Oxford Nanopore data, we assembled a full-length genome sequence of 192,237 bp.
View Article and Find Full Text PDFBluetongue virus (BTV) is an arbovirus (genus: ) that occurs worldwide. It infects domestic and wild ruminant species and can cause disease in livestock, producing high economic impact. Recently, it gained extra prominence throughout Europe, with disease occurring in regions traditionally free of BTV.
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- Bluetongue virus (BTV) is a global disease, primarily affecting ruminants like sheep, and poses significant trade and economic challenges, particularly in Australia where monitoring programs have been established.
- In 2015, BTV-5 was detected in the Northern Territory, marking its first appearance in Australia, prompting a need for genome sequencing to understand its relationship with global isolates and highlighting cross-reactivity with BTV-9.
- The study suggests revising the current serological classification of BTV strains and emphasizes ongoing surveillance for both the virus and its vectors to manage the risk of new strains entering Southeast Asia, advocating for a whole genome approach in monitoring.
In this study, an alphavirus vector platform was used to deliver replicon particles (RPs) expressing African swine fever virus (ASFV) antigens to swine. Alphavirus RPs expressing ASFV p30 (RP-30), p54 (RP-54) or pHA-72 (RP-sHA-p72) antigens were constructed and tested for expression in Vero cells and for immunogenicity in pigs. RP-30 showed the highest expression in Vero cells and was the most immunogenic in pigs, followed by RP-54 and RP-sHA-p72.
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