Publications by authors named "A Castinel"
The transition from a milk-based diet to exclusive solid feeding deeply modifies microbiota-host crosstalk. Specifically, early ingestion of plant polysaccharides would be one of the main nutritional components to drive host-microbiota-interaction. To capture the effects of polysaccharides early-life nutrition (starch vs rapidly fermentable fiber) on the holobiont development, we investigated on the one hand the gut bacteriome and metabolome and on the other hand the transcriptome of two host gut tissues.
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- DNA size fractionation is a technique used to isolate specific DNA targets from mixtures with diverse molecular weights, and microfluidics was initially considered a way to improve this process.
- The μLAF (μ-laboratory for DNA fractionation) technology enhances DNA size selection by using electrohydrodynamic migration to manipulate DNA molecules on thin films with tiny pores.
- This method can process milliliter-scale samples quickly (around 10 minutes) and effectively separate DNA in sizes ranging from 200 to 1000 base pairs, while achieving collection efficiencies of about 20-40% and improved enrichment compared to current methods.
Inspired by the production of reference data sets in the Genome in a Bottle project, we sequenced one Charolais heifer with different technologies: Illumina paired-end, Oxford Nanopore, Pacific Biosciences (HiFi and CLR), 10X Genomics linked-reads, and Hi-C. In order to generate haplotypic assemblies, we also sequenced both parents with short reads. From these data, we built two haplotyped trio high quality reference genomes and a consensus assembly, using up-to-date software packages.
View Article and Find Full Text PDFPurpose: The aim of this study was to evaluate the efficacy and safety of radiofrequency ablation (RFA) in the management of haemorrhoidal disease with 1 year's follow-up.
Method: This prospective multicentre study assessed RFA (Rafaelo) in outpatients with grade II-III haemorrhoids. RFA was performed in the operating room under locoregional or general anaesthesia.
Accurate species phylogenies are a prerequisite for all evolutionary research. Teleosts are the largest and most diversified group of extant vertebrates, but relationships among their three oldest extant lineages remain unresolved. On the basis of seven high-quality new genome assemblies in Elopomorpha (tarpons, eels), we revisited the topology of the deepest branches of the teleost phylogeny using independent gene sequence and chromosomal rearrangement phylogenomic approaches.
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