A multi-scale integrative approach to study the impact of a common pesticide, the dimethoate, on a mangrove fiddler crab Tubuca urvillei.

At land-sea interface, mangroves are likely to be exposed to pesticides due to agricultural run-offs. In Mayotte Island (Comoros archipelago, Mozambique Channel), dimethoate (DMT) is found in high concentrations in tomatoes, but no data confirm its presence in mangroves. We aimed at screening the presence of DMT in three mangroves of Mayotte at different levels (highest point above crops, village, upstream mangrove, downstream mangrove) and assessing the impact of DMT coupled with reduced salinity on mangrove crab physiology.

Evaluation of Coriolis Micro Air Sampling to Detect Volatile and Semi-Volatile Organic Compounds.

There are several analytical procedures available for the monitoring of volatile organic compounds (VOCs) in the air, which differ mainly on sampling procedures. The Coriolis micro air sampler is a tool normally designed for biological air sampling. In this paper, the Coriolis micro bio collector is used to evaluate its ability to sample organic contaminants sampling and detecting them when combined GC-MS.

Untargeted metabolomic approaches in food authenticity: A review that showcases biomarkers.

The assessment of food authenticity is a topic that gained a lot of interest at the international level. This term includes misidentification of variety, origin, production system, processing but also adulteration. These frauds all have an analytical component, and research tends to offer new analytical solutions to manage them.

Assessment of micropollutants toxicity by using a modified Saccharomyces cerevisiae model.

Environment can be affected by a variety of micropollutants. In this paper, we develop a system to assess the toxicity on an environmental sample, based on the expression of a nanoluciferase under the control of the STB5 promotor in a yeast. The STB5 gene encodes for a transcription factor involved in a pleiotropic drug resistance and in the oxidative stress response.

The Utilization of Linear Polylysine Coupled with Mechanic Forces to Extract Microbial DNA from Different Matrices.

Molecular approaches are powerful tools that are used for medical or environmental diagnoses. However, the main limitations of such a tools are that they extract low levels of DNA and they do not remove the inhibitors of polymerase chain reaction (PCR). Although the use of polycation to complex and purify DNA has been described in the literature, elution often requires a high ionic strength or pH levels not compatible with molecular analyses.