Evaluation of a commercial real-time PCR for the detection of extended spectrum β-lactamase genes.

We investigated the performance of a real-time PCR for the detection of extended spectrum β-lactamase genes in Enterobacteriaceae (Check-MDR ESBL PCR). Results from micro-arrays were considered as the gold standard. An analysis on 489 isolates resulted in a sensitivity of 98.

Development and assessment of DArT markers in triticale.

Triticale (X Triticosecale Wittm.) is a hybrid derived by crossing wheat (Triticum sp.) and rye (Secale sp.

Evaluation of a fourth-generation latex agglutination test for the identification of Staphylococcus aureus.

In this study, we evaluated a fourth-generation agglutination assay (Staph Plus; DiaMondiaL[DML]) for the rapid identification of Staphylococcus aureus. First, comparison with three third-generation assays (Slidex Staph Plus, bioMérieux; Staphaurex Plus, Murex Diagnostics; Pastorex Staph-Plus, Sanofi Diagnostics Pasteur) was performed on a predefined strain collection: 265 coagulase-negative staphylococci (CNS), 266 methicillin-resistant S. aureus (MRSA) and 262 methicillin-susceptible S.

The influence of the position of the oxygen dissociation curve on oxygen-dependent functions of the isolated perfused rat liver. I. Studies at different levels of hypoxic hypoxia.

Isolated rat livers were perfused with fresh and 2,3-DPG (2,3-diphosphoglycerate)-depleted human erythrocytes at different levels of hypoxia. The mean P50 values of the measured actual oxygen dissociation curves (O.D.