Tailored Perioperative Medicine For Frail Patients Undergoing Vascular Surgery: A Narrative Review.

Frailty is a concept that is emerging as an important tool in the preoperative assessment of patients. The incidence of frailty in vascular surgery patients is high and is expected to increase concomitantly with the aging of the population. The identification of these patients and their optimization in the perioperative period can lead to an improvement in their outcomes with a reduction in morbidity and mortality.

FRAP Assay to Trace Lipid Mixing of the Inner and Outer Leaflet of Yeast Vacuoles: Assessing the Fusion State in Live Cells.

Fluorescence recovery after photobleaching (FRAP) can be employed to investigate membrane lipid mixing of vacuoles in live budding yeast cells and distinguish the fused, hemi-fused or non-fused states of these organelles under physiological conditions. Here, we describe a protocol for labeling the outer and inner leaflets of vacuoles in live cells that allow to detect hemifusion intermediates and, thus, identify components necessary for fusion pore opening.

I-motif formation in the promoter region of the B-MYB proto-oncogene.

Understanding the mechanisms of carcinogenesis is essential to combat cancer. The search for alternative targets for anticancer therapy has gained interest, particularly when focused on upstream pathways. This strategy is particularly relevant when the encoded target proteins are known - or believed - to be "undruggable", as has been reported for the B-MYB oncogene.

Evaluation of a potential bidirectional influence of metabolic syndrome and apical periodontitis: An animal-based study.

Aim: This study aimed to explore the possible bidirectional interrelations between fructose-induced metabolic syndrome (MS) and apical periodontitis (AP).

Methodology: Twenty-eight male Wistar rats were distributed into four groups (n = 7, per group): Control (C), AP, Fructose Consumption (FRUT) and Fructose Consumption and AP (FRUT+AP). The rats in groups C and AP received filtered water, while those in groups FRUT and FRUT+AP received a 20% fructose solution mixed with water to induce MS.

Let-7b, miR-29b, and miR-125b as Potential Biomarkers for Differentiating Canine Mammary Carcinoma Histological Types.

Epigenetics is the study of changes in organisms that result from modifications in gene expression rather than alterations in the genetic code itself [...