Leachables Analysis from a Closed Connected Single-Use mAb Purification Process.

During a closed connected single-use monoclonal antibody (mAb) purification process, samples for leachables screening were gathered from two parallel processes (using different capturing chromatography), from perfusion culture to final storage bags. These samples were prepared and analyzed using screening methods for HS-GC-MS, GC-MS, LC-QToF/ESI pos and neg, to be able to identify a broad spectrum of leachables. The identified compounds were sorted into sample points from different steps of the mAb process, compared with available extractables data mapped from the process equipment used.

Long-term effects of adding biochar to soils on organic matter content, persistent carbon storage, and moisture content in Karagwe, Tanzania.

Soils require the application of biochar to improve degradation. The objective of this study was to evaluate the long-term effects of a field experiment on soil organic matter (SOM), soil organic carbon (SOC), and soil moisture content in Karagwe, Tanzania. Seven years ago, a field experiment was conducted using a Latin rectangle design with four replications.

The effects of costly telomere maintenance on lifespan - reproductive tradeoffs in sand lizards.

Telomeres are DNA-protein structures that primarily protect chromosomes and serve multiple functions of gene regulation. When cells divide, telomeres shorten and their main repair system in ectotherms - telomerase - replaces lost nucleotide complexes ((T2AG3)n in vertebrates). It remains a challenge to experimentally investigate resource requirements for telomere maintenance and its effects on lifespan-reproductive tradeoffs in the wild.

Isolated during adolescence: long-term impact on social behavior, pain sensitivity, and the oxytocin system in male and female rats.

Background: Adolescent social isolation (ASI) has profound long-term effects on behavioral and neural development. Despite this, the specific long-term impact of ASI during different adolescent stages and across sexes remain underexplored.

Methods: Our study addresses this gap by examining the effects of early- and late- adolescent social isolation on both male and female rats.

Alcohol Use Disorder-Associated DNA Methylation in the Nucleus Accumbens and Dorsolateral Prefrontal Cortex.

Background: Alcohol use disorder (AUD) has a profound public health impact. However, understanding of the molecular mechanisms that underlie the development and progression of AUD remains limited. Here, we investigated AUD-associated DNA methylation changes within and across 2 addiction-relevant brain regions, the nucleus accumbens and dorsolateral prefrontal cortex.