Publications by authors named "A C Culhane"
Background: Multiplexing single-cell RNA sequencing experiments reduces sequencing cost and facilitates larger-scale studies. However, factors such as cell hashing quality and class size imbalance impact demultiplexing algorithm performance, reducing cost-effectiveness.
Findings: We propose a supervised algorithm, demuxSNP, which leverages both cell hashing and genetic variation between individuals (single-nucletotide polymorphisms [SNPs]).
The embryonic cerebrospinal fluid (eCSF) plays an essential role in the development of the central nervous system (CNS), influencing processes from neurogenesis to lifelong cognitive functions. An important process affecting eCSF composition is inflammation. Inflammation during development can be studied using the maternal immune activation (MIA) mouse model, which displays altered cytokine eCSF composition and mimics neurodevelopmental disorders including autism spectrum disorder (ASD).
View Article and Find Full Text PDFObjectives: Physician empathy and communication skills are crucial parts of a successful emergency department (ED) interaction. This study aimed to evaluate whether these skills can be improved through a novel curriculum where interns act as patients for their senior residents during simulated ED cases.
Methods: Twenty-five residents participated in the curriculum.
Article Synopsis
- The study addresses a key challenge in treating solid tumors with CAR T cells, specifically the negative effects of the tumor microenvironment (TME).
- Researchers created a modified CAR T cell (G36-PDL1) that not only targets a protein associated with tumors (carbonic anhydrase IX or CAIX) but also secretes an antibody to counteract immune suppression.
- In tests using a mouse model of kidney cancer, the G36-PDL1 CAR T cells showed strong anti-tumor effects by enhancing immune responses and reducing suppressive cells in the TME compared to standard CAR T cells.