Publications by authors named "A Brueckner"
Molecular dynamics (MD) simulation is a powerful tool for characterizing ligand-protein conformational dynamics and offers significant advantages over docking and other rigid structure-based computational methods. However, setting up, running, and analyzing MD simulations continues to be a multi-step process making it cumbersome to assess a library of ligands in a protein binding pocket using MD. We present an automated workflow that streamlines setting up, running, and analyzing Desmond MD simulations for protein-ligand complexes using machine learning (ML) models.
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- Disproportionality analyses in pharmacovigilance are essential for detecting safety signals from individual case safety reports, but there are no clear guidelines for publishing these analyses, leading to potential misunderstandings.
- The READUS-PV statement was created to address these concerns by providing a set of 14 guidelines aimed at improving the transparency and comprehensiveness of disproportionality studies.
- By promoting better reporting standards, the READUS-PV guidelines aim to enhance the accuracy of drug safety data interpretation and support evidence-based decision-making in pharmacovigilance.
Article Synopsis
- Disproportionality analyses are key for detecting drug safety signals but are often poorly reported, prompting the creation of new guidelines called READUS-PV for better reporting practices.
- A panel of 34 international experts developed these guidelines through surveys and consensus meetings, resulting in a checklist of 32 recommendations for reporting in main articles and 12 for abstracts.
- The implementation of READUS-PV aims to enhance transparency and improve the quality of publications related to drug safety signals.
The conformational state of DNA fine-tunes the transcriptional rate and abundance of RNA. Here, we report that G-quadruplex DNA (G4-DNA) accumulates in neurons, in an experience-dependent manner, and that this is required for the transient silencing and activation of genes that are critically involved in learning and memory in male C57/BL6 mice. In addition, site-specific resolution of G4-DNA by dCas9-mediated deposition of the helicase DHX36 impairs fear extinction memory.
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