Publications by authors named "A Brisabois"

Article Synopsis
  • The study investigates the presence of antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs) in raw milk cheese samples from sheep and cows in France.
  • Analysis showed that a significant portion of the cheese samples (over 75%) contained ARGs that provide resistance to commonly used antibiotics, while more critical resistance genes were seldom found.
  • The findings suggest that while resistance to basic antibiotics is widespread, the resistance to important last-generation antibiotics is rare, indicating a mixed risk profile in raw milk cheese for antimicrobial resistance.
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Introduction: The awareness of scientists and policy makers regarding the requirement for an integrated One Health (OH) approach in responding to zoonoses has increased in recent years. However, there remains an overall inertia in relation to the implementation of practical cross-sector collaborations. Foodborne outbreaks of zoonotic diseases continue to affect the European population despite stringent regulations, evidencing the requirement for better 'prevent, detect and response' strategies.

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The French National Reference Centre for and (FNRC-ESS) detected two human clusters of 33 cases (median age: 10 years; 17 females) infected by serotype Bovismorbificans, ST142, HC5_243255 (EnteroBase HierCC‑cgMLST scheme) in September-November 2020 and of 11 cases (median age: 11 years; seven males) infected by serotype 4,12:i:-, ST34, HC5_198125 in October-December 2020. Epidemiological investigations conducted by Santé publique France linked these outbreaks to the consumption of dried pork sausages from the same manufacturer.  Bovismorbificans and  4,12:i:- were isolated by the National Reference Laboratory from different food samples, but both strains were identified in a single food sample only by qPCR.

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From a global view of antimicrobial resistance over different sectors, seafood and the marine environment are often considered as potential reservoirs of antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs); however, there are few studies and sparse results on this sector. This study aims to provide new data and insights regarding the content of resistance markers in various seafood samples and sources, and therefore the potential exposure to humans in a global One Health approach. An innovative high throughput qPCR screening was developed and validated in order to simultaneously investigate the presence of 41 ARGs and 33 MGEs including plasmid replicons, integrons, and insertion sequences in Gram-negative bacteria.

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We report here the closed genome sequence of one Salmonella enterica subsp. serovar Bovismorbificans strain isolated from dried pork sausage consumed by a patient suffering from salmonellosis.

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