Publications by authors named "A Brenet"
It has been known for a long time that epileptic seizures provoke brain neuroinflammation involving the activation of microglial cells. However, the role of these cells in this disease context and the consequences of their inflammatory activation on subsequent neuron network activity remain poorly understood so far. To fill this gap of knowledge and gain a better understanding of the role of microglia in the pathophysiology of epilepsy, we used an established zebrafish Dravet syndrome epilepsy model based on Scn1Lab sodium channel loss-of-function, combined with live microglia and neuronal Ca imaging, local field potential (LFP) recording, and genetic microglia ablation.
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- Organophosphorus (OP) compounds are a significant global public health issue, causing millions of poisonings and over 200,000 deaths annually due to their severe effects on the nervous system.
- Using a zebrafish model, researchers demonstrated that diisopropylfluorophosphate (DFP), a toxic OP, leads to enzyme inhibition, paralysis, neuron overactivity, increased apoptosis, and potential long-term cognitive damage.
- The study found that DFP exposure alters the balance of synaptic activity in neurons, which suggests that the zebrafish model can help deepen understanding of OP toxicity and aid in finding new antidotes.
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- Succinate dehydrogenase inhibitors (SDHIs) are widely used fungicides that block an essential enzyme in both fungi and human cells, raising concerns about their specificity and health risks for humans.
- A recent study focused on bixafen, a new-generation SDHI, using zebrafish embryos to assess its effects on neurodevelopment.
- The results showed that bixafen caused microcephaly and impaired motor neuron development, indicating significant neurotoxic effects and highlighting the need for better safety evaluations of SDHIs in agriculture.
In all animal species, oxygen consumption is a key process that is partially impaired in a large number of pathological situations and thus provides informative details on the physiopathology of the disease. In this study, we describe a simple and affordable method to precisely measure oxygen consumption in living zebrafish larvae using a spectrofluorometer and the MitoXpress Xtra Oxygen Consumption Assay. In addition, we used zebrafish larvae treated with mitochondrial respiratory chain inhibitors, antimycin A or rotenone, to verify that our method enables precise and reliable measurements of oxygen consumption.
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