Demonstration of Sindbis virus antigen in Lowicryl-embedded cultured cells by immunogold staining.

Eight monoclonal antibodies (MAbs) were tested for the use in immunogold staining of Sindbis virus (SIN) antigen in cultured Baby Hamster kidney (BHK) cells. The antibodies were directed against the capsid (C) protein (5) or against the glycoprotein E1 (3), respectively. Four out of five anti-C MAbs and two out of three anti-E1 Mabs reacted on frozen sections, whereas two of the anti-C antibodies and none of the anti-E1 antibodies reacted on Lowicryl K4M-embedded cells.