Prolactin gene expression in human myometrial smooth muscle cells is induced by cyclic adenosine 3',5'-monophosphate.

We have previously characterized PRL production in human myometrial tissue maintained in explant culture. Here we describe PRL gene expression and its regulation in smooth muscle cells isolated from normal human myometrium. Onset of PRL secretion occurred spontaneously after several days in culture and increased over time without exogenous stimulation.

Effects of clomiphene citrate stimulation on endometrial structure in infertile women.

Investigations into the effect of clomiphene citrate stimulation on endometrial differentiation were retrospectively performed in the mid-luteal phase on 21 patients undergoing ovulation induction prior to artificial insemination. A high incidence of irregular endometrial development was seen using morphological and morphometric criteria; in addition, increasing stromal oedema after clomiphene citrate treatment was observed. Measurable differences in glandular development (i.

Identification of macroprolactin in a patient with asymptomatic hyperprolactinemia as a stable PRL-IgG complex.

We characterize the molecular form of PRL in a female patient with asymptomatic, idiopathic hyperprolactinemia. Size exclusion chromatography revealed that PRL was present exclusively in the form of macroprolactin (> 200 kD mol wt). By immunoaffinity purification, Western blot analysis, and binding studies the molecule was identified as a PRL-IgG complex of extremely high stability.

Modulation of prolactin secretion in human myometrium by cytokines.

Prolactin (PRL) secretion and PRL mRNA expression in human myometrial explant cultures was inhibited by the addition of human placental conditioned medium (HPCM). After 3 days treatment with 5% HPCM PRL secretion was reduced to 24% of control values (P < 0.001).