Live cell cytoplasm staining and selective labeling of intracellular proteins by non-toxic cell-permeant thiophene fluorophores.

A structurally correlated series of cell-permeant thiophene fluorophores, characterized by intense green or red fluorescence inside live mouse embryonic fibroblasts, was developed. The fluorophores displayed rapid internalization, excellent retention inside the cells, and high optical stability in the cytosolic environment and did not alter cell viability and reproducibility. Depending on the molecular structure, they experienced distinct fate inside the cells: from bright and lasting staining of the cytoplasm to selective tagging of a small set of globular proteins.

Fluorodeoxyglucose positron emission tomography may aid the diagnosis of aggressive primary prostate cancer: A case series study.

Recent evidence has shown that positive results may be observed for fluorodeoxyglucose-positron emission tomography (FDG-PET) in undifferentiated, biologically aggressive and metastatic tumors. The present study describes a case series of six patients with normal prostate-specific antigen (PSA) serum levels who underwent FDG-PET due to other causes. Positive PET results were observed at the prostate and the patients were subsequently diagnosed with high-risk prostate cancer.

Push-pull amino succinimidyl ester thiophene-based fluorescent dyes: synthesis and optical characterization.

The design and synthesis of new fluorescent dyes with emission range at 490-650 nm are described. Their structural and electronic properties have been characterized by both experimental techniques and quantum-chemical calculations. The chromophores are donor-π-bridge-acceptor push-pull compounds with a π bridge of phenyl and thiophene rings and their combination.

Silyloxyazadienes: one intermediate and two competitive pericyclic reactions.

The two competing mechanisms in the reaction of 3-trialkylsilyloxy-2-aza-1,3 dienes to form beta-lactams through a [2+2] electrocyclic ring closure or tetrahydrooxazinan-4-ones via a [4+2] hetero-Diels-Alder reaction were studied using Density Functional computations. Although the [2+2] and [4+2] mechanisms are typical of dienes, their competition, starting from the same diene intermediate, has not yet been observed and analyzed. This competition is governed by a delicate interplay between temperature and substituents at the diene and dienophile, respectively.

Alkylenesulfanyl-bridged bithienyl cores for simultaneous tuning of electronic, filming, and thermal properties of oligothiophenes.

DPY and DPE alkylenesulfanyl-bridged bithienyls were prepared by a highly effective ring-closing reaction via arylalkylsulfonium intermediate and used as inner cores in oligothiophenes. HOMO-LUMO energy levels, conformational flexibility, and intrinsic asymmetry of the cores are reflected in the electronic, film-forming, and thermal properties of the corresponding oligomers.